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Tissue Culture Inserts

ThinCertTM
6, 12 and 24 Well Cell Culture Inserts for Multiwell Plates

For advanced cell and tissue culture applications, Greiner Bio-One offers an extensive family of membrane supports - ThinCertTM. Combining 6 different membrane specifications (pore size and density) in geometries to fit 6,12 and 24 well plates, the ThinCertTM  cell culture inserts are suitable for a wide range of applications including transport, secretion and diffusion studies, migrational experiments, cytotoxicity testing, co-cultures, trans epithelial electric resistance (TEER) measurements, as well as primary cell culture.
ThinCertTM cell culture inserts are compatible with standard CELLSTAR® multiwall plates from Greiner Bio-One, and are pre-packed together with the requisite number of plates. The automated production process includes double optical control of each insert produced, ensuring that any biological contamination is avoided. The sterility of the single blisterpacked inserts and multiwall plates is ensured by irradiation.

ThinCertTM cell culture inserts are produced from high-grade clear polystyrene housings, and polyethylene terephthalate (PET) capillary pore membranes. Both materials, polystyrene and PET, are USP class VI certified and cell culture compatible. The coupling between the housing and the membrane is achieved using an automated process which produces an extremely strong and robust seal without compromising or weakening the membrane in any way. The membranes undergo a physical surface treatment to optimise cellular adherence and growth characteristics. All the capillary pores in a membrane exhibit a high degree of uniformity in diameter. This uniformity ensures reliable and consistent exchange rates between the two compartments and thus provides reproducibility when conducting multiple experiments.

 Which Membrane to use?

General Aspects:

  • Small pore sizes (0.4 and 1 μm in diameter) for co-cultivation as well as for transportation, secretion, and diffusion studies
  • Larger pore sizes (3 and 8 μm in diameter) for migration and invasion studies
  • Transparent membranes (in general low pore density) suitable for light and electron microscopy
  • Translucent membranes (in general high pore density) suitable for electron microscopy, TEER, and transport assays

Suitability chart of the different membrane types:

  0,4 μm transparent
0,4 μm translucent
1,0 μm transparent 3,0 μm transparent 3,0 μm translucent
8,0 μm translucent
Light microscopy
+ - + + - +
Electron microscopy
+ + + + + +
Fluorescence microscopy
+ + + + + +
Immunocytochemistry + + + + + +
Trans epithelial electric resistance (TEER) + +* + + +* +*
Transport and uptake studies
- + + + + +
Co-cultivation + + + - - -
Cell retention
+ + + - - -
Migration/chemotaxis/metastasiation - - - + + +

+* very suitable
+ suitable
- not suitable

 

Further Information/Application Notes:




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