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Base data

  • Cat.-No.: 627870
  • Product description:
  • Packaging weight: 0,40 kg
  • Packaging dimension: 195 x 142 x 122 mm
  • sales unit: 40 piece / carton

Detailed information

CELLview cell culture dish

  •   Advantages:
    • Subdivided version enables simultaneous multiplex analysis
    • Embedded glass bottom for maximal planarity
    • Additional TC surface treatment and Advanced TC surface modification available
  • Applications:
    • Phase contrast microscopy
    • Fluorescence microscopy
    • Confocal microscopy
    • Live cell imaging
    • Differential interference contrast microscopy
    • Polarised light microscopy
    • Fluorescence-in-situ-hybridisation (FISH)
  • Free of detectable DNase, RNase, human DNA
  • Non-pyrogenic
  • Non-cytotoxic
  • Glass bottom
  • No. of compartments: 4
  • ø 35 mm, height 10 mm
  • Growth area: 1.9 cm²/compartment
  • Total volume: 1.5 ml/compartment
  • Working volume: 0.1 ml/0.5 ml (0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment)
  • TC surface treatment
  • Sterile

 Quantity per bag/case: 10/40

Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
  • Video 1 - control (no drugs added)
    In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.

  • Video 2 - Nocodazole added, final concentration 10 µM
    Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.

  • Video 3 - Latrunculin B added, final concentration 1 μM
    Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
    rearrangement was completed.

  • Video 4 - Brefeldin A added, final concentration 5 μg/ml
    Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.

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