FAQ - CELLSTAR® Culture Flasks
1. What are GREINERs TC treated products coated with?
Our CellStar TC Treated products are not coated with any chemical or biological product. Rather, the surface is physically modified to make it suitable for cell culture applications. Plasticware coated with extracellular matrix or synthetic proteins is available in our CellCoat line of products. Standard catalog products include Collagen I, Poly-D-Lysine, and Poly-L-Lysine.
2. Does GREINER have TC products coated with proteins other than Collagen type I, Poly- D-Lysine and Poly-L-Lysine?
Yes. GREINER will coat its TC products with Laminin or Fibronectin upon special request
3. What are the sources of the proteins that GREINER uses to coat its CellCoat TC
Collagen I: Rat Tail Tendon Poly-D and Poly-L-Lysine: Synthetic Laminin: murine Engelbreth-Holm-Swarm tumor cells Fibronectin: human plasma
4. Does GREINER offer filter caps for all its TC Flasks?
Yes. Filter caps are available for all our TC flasks.
5. What is the difference between High profile and Low profile T182 flasks?
The total volume of the low profile T182 flask is 550 mL, while the total volume of the high profile T182 flask is 650 mL. The high profile is available as a TC treated flask or a suspension (non-treated) flask. The low profile is only available TC treated. The growth (surface) area is the same for both: 182 cm2. However, the sides of the flask (when lying flat) are taller on the high profile than on the low profile flask. Therefore, the high profile suspension flask allows for a greater volume of liquid and, cells. The high profile TC treated flasks would allow for a greater volume of media to feed quickly growing cells. However, the low profile flasks take up less room in the incubator.
6. What is the difference between vented and non-vented lids?
Vented lids are manufactured so they sit slightly higher on the rim of the dishes. This allows uniform gas exchange within the dish. Non-vented lids sit flush with the rim of the dish, preventing any significant gas exchange. Filter caps on flasks and roller bottles are also referred to as vented; the filter allows gas exchange, while preventing contamination.