FAQ - HTS/Microplaten
1. Is lumox suitable for readings in the ultraviolet (UV) range?
Yes. In addition, to low autofluorescence, lumox offers excellent transmission qualities, especially compared to standard plastic or glass-bottom plates in the wavelength range from 200 to 300 nm.
2. What is the temperature resistance range of lumox?
The lumox film can be used at temperatures up to 50°C, and down to cryopreservation in the vapor phase of LN2
3. Are GREINER´s microplates autoclavable?
Only the microplates made of polypropylene are autovlavable. However, we do offer sterile polystyrene microplates that do not require autoclaving.
4. Can I use TC treated plates for EIA?
It is not recommended. TC treatment of polystyrene produces a hydrophilic, negatively charged surface. This allows molecules with a greater affinity for the surface to replace molecules with a lesser affinity. Therefore, non-specific background is hard to control.
5. When should I use a white or black plate?
The colour of the plate is dependent on the type of assay being performed. Typically white plates are used for luminescent applications and black plates are used for fluorescent applications. The opacity of these plates is designed to reduce cross talk between the wells.
6. When would I use a V-bottom or a U-bottom microplate instead of a flat bottom?
U-bottom plates are ideally suited for qualitative applications when through mixing is necessary. V-bottom plates are typically used to store small volumes when maximum recovery is necessary. Flat bottom plates are excellent for optical measurements and ideal for microscopic examination.
7. What is the advantage of a mClear plate over a standard polystyrene plate?
mClear plates have increased sensitivity down to 340 nm due to a reduction in background. The bottom of the plate is made from a thin film or polystyrene equalling 200 mm for a 96 and 384 well formats and 75 mm for the 1536 well format.
8. Does GREINER have a microplate for measurement of DNA?
Yes. They are our UVStar microplates available in 96 and 384 well formats.
9. What is the difference between µClear and UVStar® microplates?
µClear microplates have clear bottoms of ultra thin polystyrene films. They have extended wavelength ranges down to 340 nm, increased sensitivity, and reduced background. UVStar® microplates improve upon µClear capabilities. Their ultra thin film is a proprietary resin that extends the wavelength range down to 200 nm. They are highly recommended for measuring protein and DNA concentrations at 280 nm and 260 nm.
10. What is the difference between high and medium binding plates?
GREINER manufactures both medium and high binding microplates. Medium binding microplates have an approximate binding capacity 200 ng/mm² of lgG as assessed by competitive EIA, while high binding plates have a capacity of approximately 600ng/mm² of lgG. In general, high binding plates are recommended for EIA applications. The improved loading density in high binding plates produces more sensitive assays. However, as with any new assay, it is best to compare medium and high binding plates to determine which is best.