FAQ (veelgestelde vragen) - Cel- en Weefselkweek
1. What adhesive is used to affix flexiPERM?
No glues or adhesives are required to affix flexiPERM to clean, smooth surfaces such as glass or plastic.
2. How do I re-sterilize and recondition flexiPERM for re-use?
Re-usable flexiPERM may be re-sterilized using by alcohol immersion or by autoclaving. Please refer to the package insert for detailed instructions on sterilization by both methods and for additional use and reconditioning recommendations.
3. Does flexiPERM come with a lid or cover?
No. The lid or cover of the culture vessel, such as a TC dish, plate or quadriPERM, serves as the cover for the flexiPERM during the culture phase.
5. Can the miniPERM Production Module be autoclaved?
No. Damage to critical components such as the dialysis membrane could result from autoclaving. The Production Module is intended to be discarded after the bioreactor run is completed
6. Can the miniPERM Nutrient Module be autoclaved?
Yes. The properly prepared Nutrient Module can be autoclaved and re-used. Wear from repeated autoclaving will vary from lab to lab, so no claim is made regarding how many times the Nutrient Module may be successfully autoclaved.
7. Do I have to use the cellPROTECT or antiFOAM a?
The result you obtain with your application will best determine whether you choose to use or discontinue use of either cellPROTECT or antiFOAM a.
8. Do cellPROTECT or antiFOAM a contain any animal products or proteins that may interfere with purification?
No. While the exact formulae are proprietary, neither product contains any animal products or proteins.
9. Can miniPERM be used without rotation?
No. The design of miniPERM requires rotation especially for suspension culture and even anchorage dependent cells for desirable results to be achieved.
10. Can I use a standard roller apparatus, such as used for roller bottles, to rotate miniPERM?
No. While the miniPERM may fit on your roller apparatus, most roller racks do not rotate at sufficiently high RPM, or have the range of RPM needed for optimal results with a wide variety of cell types. However, the flexible Universal Turning Device can also accommodate roller bottles.
11. How do I remove my slides from quadriPERM?
quadriPERM has a concave notch or indentation molded into the base of the plate at one end of each chamber. A tool such as an inoculating needle or forceps can be inserted under the slide via notch to lift or grab the slide.
12. Is quadriPERM TC treated?
No. quadriPERM is sterile but not TC treated for culture od adherent or anchorage dependent cells. Culture of adherent cells in quadriPERM should be performed on properly prepared glass or plastic slide.
13. What kind of membrane does GBO use in Filter caps for Tissue culture flasks and Roller bottles?
For Tissue Culture Flasks and Roller bottles, GBO is using a hydrophobic capillary pore membrane. The basic material consists of PET that is covered with a thin layer of PTFE. The PTFE is responsible for the hydrophobic properties of the membrane. It is produced in a two-step process. Membranes manufactured by this process are known for their precisely determined pore size (0.2 µm for Tissue Culture products), shape and density. First noble gas ions are passing the membrane in an electric field. During this first step, the polyester chains become brittle. The tracks are finished by incubation in an etch bath. The ions pass the membrane in a slightly scattered way. For this reason two different tracks will never unify to one single pore and a precise pore size of 0.2 µm can be guaranteed. The capillary pore membrane represents a secure contamination barrier, made out of a biological inert material, which also guarantees an optimal gas exchange.
14. Is the membrane in Filter caps for Tissue culture flasks and roller bottles permeable for liquids?
No. The hydrophobic properties of the capillary pore membrane prevent liquid to stick to the membrane. The so-called water entrance pressure, a physical property of the membrane, amounts over 2.5 bars (this corresponds to a water column of 25 m). The membrane does neither wet with media nor does liquid leak through the pores even in case of accidentally dropping the flask. Functions like the high gas exchange and contamination barrier are remaining even in the described situation.
15. Does the gas exchange through the membrane as well as the contamination barrier function remain after accidentally wetting the membrane with medium?
Yes. The gas exchange as well as the contamination barrier remains after accidentally wetting the membrane. Due to the hydrophobic surface, the media does not stick to the membrane.
16. What is the function of condensation rings of lids of tissue culture treated Multiwellplates?
Lids with condensation rings reduce evaporation and decrease the risk of contamination between the wells.
17. Are tissue culture products tested for DNase, RNase as well as for endotoxins?
Yes, all tissue culture products are DNase-and RNase- free and non-pyrogenic. Testing of DNase/RNase is performed by PCR. Even low amounts of contaminants can be detected by this sensitive method. Contingently contaminants with endotoxins are detected by FDA admitted LAL-Tests (Limulus Amobocytes Lysate). The detection limit for this test is >0.06 EU/ml.
18. Does Greiner offer filter caps for all its TC flasks?
Yes. Filter caps are available for all our TC flasks.
19. What is the difference between High profile and Low profile T175 flasks?
The total volume of the low profile T175 flask is 550ml, while the total volume of the high profile T175 flask is 650 ml. The high profile is available as a TC treated flask or a suspension (non-treated) flask. The low profile is only available TC treated. The growth (surface) area is the same for both: 182cm². However, the sides of the flask (when lying flat) are taller on the high profile than on the low profile flask. Therefore, the high profile suspension flask allows for a greater volume of media.
20. What is the difference between vented and non vented lids?
Vented lids are manufactured so they sit slightly higher on the rim of the dishes. This allows uniform gas exchange within the dish. Non-vented lids sit flush with the rim on the dish, preventing any significant gas exchange.
21. What is the maximum speed at which GREINER 15 mL centrifuge tubes can be spun?
The 15 ml polypropylene tubes can be safely spun up to 13,000 RCF in a supported rotor and 3,500 RCF in an non supported rotor. The 50 ml polypropylene can be safely spun up to 9,500 RCF in a supported rotor and 3,200 RCF in an non-supported rotor.
22. What is the difference between a GREINER polystyrene roller bottle and a GREINER PET roller bottle?
GREINER´s polystyrene bottles are sterile AND TC treated, while the PET bottles are sterile, but NOT TC treated. TC treatment allows a wide variety of cell types to adhere to the surface of the roller bottle and grow well, while only some hardy cell lines will adhere to the PET roller bottles. PET roller bottles are ideal for suspension cultures. The PET roller bottles are very sturdy, more flexible, and more resistant to cracking than polystyrene bottles
23. Can my roller bottles be shipped by air?
The PET roller bottles CAN NOT be shipped by air. They are sensitive to changes in air pressure and may implode if the cabin loses pressure. The polystyrene bottles can be shipped by air.
24. What are GREINER´s TC treated products coated with?
Our CellStar® treated products are not coated with any chemical or biological product. Rather, the surface is physically modified to make it suitable for cell culture applications. Plasticware coated with extracellular matrix or synthetic proteins is available in our CellCoat® line of products. Standard catalog products include Collagen I, Poly-D-Lysine and Poly-L-Lysine.